IL-17 Protein (His tag)

Produktnummer ABIN7320461

Dieses Recombinant IL-17-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für IL-17 Protein (His tag) (ABIN7320461)

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: Cell Culture (CC)

Reinheit: > 98 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses IL-17 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse Interleukin-17/IL-17 Protein

Sequenz: Thr 22-Ala158

Produktmerkmale: A DNA sequence encoding the mature form of mouse IL17a (Q62386) (Thr 22-Ala158) was expressed and purified.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to induce IL-6 secretion in 3T3 cells. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant mouse IL-17A is > 1 x 10⁶ IU/mg.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 4.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Andere Bezeichnung: Interleukin-17/IL-17

Hintergrund: IL-17CTLA,Cytotoxic T-lymphocyte-associated antigen,IL-17Acytotoxic T-lymphocyte-associated protein,CTLA8cytotoxic T-lymphocyte-associated serine esterase,CTLA 8,CTLA8cytotoxic T-lymphocyte-associated serine esterase 8,Ctla9,IL-17A,IL-17Acytotoxic T-lymphocyte-associated protein 8,IL-17CTLA-8,Interleukin-17A,IL-17,CTLA-8,Il17a,Cytotoxic T-lymphocyte-associated antigen 8,Ctla8,Il17,CTLA8,CTLA-8,CTLA8cytotoxic T-lymphocyte-associated serine esterase 8,Cytotoxic T-lymphocyte-associated antigen 8, IL17,IL-17,IL17A,IL-17A,IL-17Acytotoxic T-lymphocyte-associated protein 8,IL-17CTLA-8,IL17, also known as IL17a, is a cytokine belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Molekulargewicht:

Calculated MW: 15.9 kDa

Observed MW: 17 kDa

Gen-ID: 16171

UniProt: Q62386