HDAC4 Protein (AA 612-1084)

Produktnummer ABIN7317483

Recombinant HDAC4-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für HDAC4 Protein (AA 612-1084) (ABIN7317483)

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 90 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 612-1084

Verwendungszweck: Recombinant Human HDAC4 Protein (aa 612-1084)

Sequenz: Met612-Leu1084

Produktmerkmale: A DNA sequence encoding the human HDAC4 (Met612-Leu1084) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris,500 mM NaCl, pH 7.4, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Andere Bezeichnung: HDAC4

Hintergrund: AHO3,BDMR,HA6116,HD4,HDAC-4,HDAC-A,HDACA,HDAC4 (histone deacetylase 4), belongs to class II of the histone deacetylase/acuc/apha family. Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general, they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs, classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions and by the class to which they belong. HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents. HDAC4 possesses histone deacetylase activity and represses transcription when tethered to a promoter. It does not bind DNA directly, but through transcription factors MEF2C and MEF2D. HDAC4 seems to interact in a multiprotein complex with RbAp48 and HDAC3.

Molekulargewicht:

Calculated MW: 50.9 kDa

Observed MW: 51 kDa

Gen-ID: 9759

NCBI Accession: NP_006028

UniProt: P56524

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process