K-RAS Protein (AA 2-185) (His tag)

Produktnummer ABIN7319807

Dieses Recombinant K-RAS-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für K-RAS Protein (AA 2-185) (His tag) (ABIN7319807)

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 2-185

Aufreinigungstag / Konjugat: Dieses K-RAS Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human KRAS(G12C, N-6His)

Sequenz: Thr2-Cys185(Gly12Cys)

Produktmerkmale: Recombinant Human GTPase Kras is produced by our E.coli expression system and the target gene encoding Thr2-Cys185(Gly12Cys) is expressed with a 6His tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Andere Bezeichnung: KRAS

Substanzklasse: Viral Protein

Hintergrund: CFC2,KRAS2,Ki-Ras,RASK2,c-K-ras,K-Ras belongs to the small GTPase superfamily, Ras family. As other members of the Ras family, K-Ras is a GTPase and is an early player in many signal transduction pathways. It is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. K-Ras functions as a molecular on/off switch. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner. Besides essential function in normal tissue signaling, the mutation of a K-Ras gene is an essential step in the development of many cancers. Several germline K-Ras mutations have been found to be associated with Noonan syndrome[4] and cardio-facio-cutaneous syndrome. Somatic K-Ras mutations are found at high rates in Leukemias, colon cancer, pancreatic cancer and lung cancer.

Molekulargewicht:

Calculated MW: 23.6 kDa

Observed MW: 26 kDa

Gen-ID: 3845

UniProt: P01116