Granulin Protein (GRN) (His tag)

Produktnummer ABIN7320292

Dieses Recombinant Granulin-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für Granulin Protein (GRN) (His tag) (ABIN7320292)

Target: Granulin (GRN)

Protein-Typ: Recombinant

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 90 % as determined by SDS-PAGE

Produktdetails

Aufreinigungstag / Konjugat: Dieses Granulin Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse Granulin/GRN/Progranulin Protein (His Tag)

Sequenz: Met 1-Leu 589

Produktmerkmale: A DNA sequence encoding the mouse GRN (NP_032201.2) (Met 1-Leu 589) was fused with a polyhistidine tag at the C-terminus.

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: Granulin (GRN)

Andere Bezeichnung: Granulin/GRN/Progranulin

Substanzklasse: Viral Protein

Hintergrund:

Background: Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. Granulin expression inhibited Tat transactivation, and tethering experiments showed that this effect was due, at least in part, to a direct action on cyclin T1 in the absence of Tat.

Synonym: epithelin,Pgrn

Molekulargewicht: 63 kDa

NCBI Accession: NP_032201