GRK6 Protein (His-GST)

Produktnummer ABIN7317064

Dieses Recombinant GRK6-Protein wird in Baculovirus infected Insect Cells exprimiert.

Kurzübersicht für GRK6 Protein (His-GST) (ABIN7317064)

Target: GRK6 (G Protein-Coupled Receptor Kinase 6 (GRK6))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 93 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses GRK6 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human GRK6/GPRK6 Protein (His & GST Tag)

Sequenz: Met 1-Arg 589

Produktmerkmale: A DNA sequence encoding the human GRK6 isofrom B (P43250-2) (Met 1-Arg 589) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 7 nmol/min/mg using casein as substrate.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 2 mM GSH, 0.5 mM PMSF, pH 7.4

Konservierungsmittel: PMSF

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: GRK6 (G Protein-Coupled Receptor Kinase 6 (GRK6))

Andere Bezeichnung: GRK6/GPRK6

Hintergrund: GPRK6,G protein-coupled receptor kinase 6, also known as G protein-coupled receptor kinase GRK6, GRK6 and GPRK6, is a lipid-anchor protein which belongs to theprotein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. GRK6 / GPRK6 contains oneAGC-kinase C-terminal domain, oneprotein kinase domain and oneRGS domain. This protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described. GRK6 / GPRK6 is widely expressed. GRK6 / GPRK6 silencing causes suppression of signal transducer and activator of transcription 3 (STAT3) phosphorylation associated with reduction in MCL1 levels and phosphorylation, illustrating a potent mechanism for the cytotoxicity of GRK6 inhibition in multiple myeloma (MM) tumor cells. GRK6 also appears to be involved in responses to morphine. Inhibition of GRK6 represents a uniquely targeted novel therapeutic strategy in human multiple myeloma.

Molekulargewicht:

Calculated MW: 95.1 kDa

Observed MW: 85 kDa

Gen-ID: 2870

UniProt: P43250

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Negative Regulation of Transporter Activity