DYRK3 Protein (His-GST)

Produktnummer ABIN7317092

Recombinant DYRK3-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für DYRK3 Protein (His-GST) (ABIN7317092)

Target: DYRK3 (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 3 (DYRK3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 85 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses DYRK3 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human DYRK3/REDK Protein (His & GST Tag)

Sequenz: Met 1-Ser 588

Produktmerkmale: A DNA sequence encoding the human DYRK3 isoform 1 (O43781-1) (Met 1-Ser 588) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 22 nmol/min/mg using synthetic DYRKtide peptide (RRRFRPASPLRGPPK) as substrate.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 0.5 mM PMSF, 10 % glycerol, pH 8.0

Konservierungsmittel: PMSF

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: DYRK3 (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 3 (DYRK3))

Andere Bezeichnung: DYRK3/REDK

Hintergrund: DYRK5,RED,REDK,hYAK3-2,Dual specificity tyrosine-phosphorylation-regulated kinase 3, also known as Regulatory erythroid kinase, REDK and DYRK3, is a nucleus protein which belongs to theprotein kinase superfamily, CMGC Ser/Thr protein kinase family and MNB/DYRK subfamily. DYRKs are an emerging family of dual-specificity kinases that play key roles in cell proliferation, survival, and development. DYRK3 contains oneprotein kinase domain. Isoform 1 and isoform 2 of DYRK3 are highly expressed in testis and in hematopoietic tissue such as fetal liver, and bone marrow. Isoform 2 of DYRK3 is the predominant form in testis. Isoform 1 of DYRK3 is the predominant form in fetal liver and bone marrow. Isoform 1 and isoform 2 are present at low levels in heart, pancreas, lymph node, and thymus. DYRK3 is a negative regulator of EPO-dependent erythropoiesis. It may place an upper limit on red cell production during stress erythropoiesis. DYRK3 inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells. It may also act by regulating CREB/CRE signaling. DYRK3 proved to effectively inhibit NFAT (nuclear factor of activated T cells) transcriptional response pathways and to co-immunoprecipitate with NFATc3. DYRK3 attenuates (and possibly apportions) red cell production selectively during anemia.

Molekulargewicht:

Calculated MW: 93.5 kDa

Observed MW: 80 kDa

Gen-ID: 8444

UniProt: O43781

Pathways: Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha