DUSP14 Protein (His-MBP)

Produktnummer ABIN7317376

Dieses Recombinant DUSP14-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für DUSP14 Protein (His-MBP) (ABIN7317376)

Target: DUSP14 (Dual Specificity Phosphatase 14 (DUSP14))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 88 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses DUSP14 Protein ist gelabelt mit His-MBP.

Verwendungszweck: Recombinant Human DUSP14/MKP-6 Protein (His & MBP Tag)

Sequenz: Met 1 -His 191

Produktmerkmale: A DNA sequence encoding the human DUSP14 (O95147) (Met 1 -His 191) was fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.

Sterilität: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: DUSP14 (Dual Specificity Phosphatase 14 (DUSP14))

Andere Bezeichnung: DUSP14/MKP-6

Hintergrund: MKP-L,MKP6,Dual specific phosphatase 14 / MAP-kinase phophatase-6 (DUSP14 / MKP6) is a member of Dual-specificity phosphatases that is a subclass of protein tyrosine phosphatases (PTP) families that can dephosphorylate bothe phosphotyrosine and phosphoserine / phosphothreonine residues in substrates. Unlike many other DUSPs, DUSP14 only contains a catalytic domain within the C-terminal region. In signal transduction, DUSP14 has been considered as negative regulator of the mitogen-activated protein kinase (MAPK) / extracellular signal-regulated kinase 1 / 2 (ERK 1 / 2) pathway. DUSP14 phosphatase activity has been confirmed to be inhibited by PTP inhibitor IV. PTP inhibitor binds to the catalytic site of DUSP14. PTP inhibitor IV effectively and specifically inhibited DUSP14-mediated dephosphorylation of JNK, a member of the mitogen-activated protein kinase (MAPK) family through dephosphorylation of both the Ser / Thr and Tyr residues of MAPKs.

Molekulargewicht:

Calculated MW: 65.0 kDa

Observed MW: 60 kDa

Gen-ID: 11072

UniProt: O95147