Chitotriosidase 1 Protein (His tag)

Produktnummer ABIN7195100

Recombinant Chitotriosidase 1-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für Chitotriosidase 1 Protein (His tag) (ABIN7195100)

Target: Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 94 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses Chitotriosidase 1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Chitotriosidase/CHIT1 Protein (His Tag)

Sequenz: Met 1-Asn 466

Produktmerkmale: A DNA sequence encoding the human CHIT1 (NP_003456.1) (Met 1-Asn 466) precursor was fused with a polyhistidine tag at the C-terminus.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 12.5 mM Tris, 75 mM NaCl, 50 % glycerol, pH 7.5

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))

Andere Bezeichnung: Chitotriosidase/CHIT1

Hintergrund:

Background: Chitotriosidase, also known as Chitinase-1 and CHIT1, is a member of the glycosyl hydrolase 18 family and Chitinase class II subfamily. It is a member of the mammalian chitinase family, structurally homologous to chitinases from other species, is synthesized and secreted by specifically activated macrophages. Chitotriosidase is a polymer of N-acetylglucosamine. Serum and plasma chitotriosidase activity is usually measured as the first step in diagnosis of Gaucher disease. Monitoring chitotriosidase activity is widely used during treatment of this pathology by enzyme replacement therapy. Its elevated plasma level reflects gradual intralysosomal accumulation in Gaucher cells (lipid-loaded macrophages). Macrophages overloaded by the enzyme accumulated in lysosomal material (lipids) were shown to secrete chitotriosidase, its increased expression was noted in several lysosomal storage diseases and atherosclerosis. In addition to lipid storage disorders, where Chit activity has longer been used as a marker of disease activity and therapeutic response, elevation of plasma Chit may occur in hematological disorders with storage of erythrocyte membrane breakdown products as thalassemia and different systemic infectious diseases sustained by fungi and other pathogens. Recently, increased Chit activity was demonstrated in CNS from patients with different neurological disorders. Chitotriosidase is believed to play a role in mechanisms of immunity and protection against chitin-containing pathogens.

Synonym: Chitotriosidase-1, Chitinase-1, CHIT1,CHI3,CHIT,CHITD

Molekulargewicht: 50.8 kDa

NCBI Accession: NP_003456