CD47 Protein (CD47) (His tag)

Produktnummer ABIN7319534

Dieses Recombinant CD47-Protein wird in Human Cells exprimiert.

Kurzübersicht für CD47 Protein (CD47) (His tag) (ABIN7319534)

Target: CD47

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Human Cells

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses CD47 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human CD47/IAP Protein (His Tag)(Active)

Sequenz: Gln19-Pro139

Produktmerkmale: Recombinant Human CD47 is produced by our Mammalian expression system and the target gene encoding Gln19-Pro139 is expressed with a 6His tag at the C-terminus.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human SIRPA-Fc(Cat: PKSH033061) at 10μg/ml(100 μl/well) can bind Human CD47-His. The ED50 of Human CD47-His is 4.18 ng/ml .

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-Citrate, 150 mM NaCl, pH 8.0.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: CD47

Andere Bezeichnung: CD47/IAP

Hintergrund:

Background: CD47(Integrin-Associated Protein,IAP) is a 40 - 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes.

Synonym: Leukocyte Surface Antigen CD47, Antigenic Surface Determinant Protein OA3, Integrin-Associated Protein, IAP, Protein MER6, CD47, MER6

Molekulargewicht: 14.76 kDa

UniProt: Q08722