CD47 Protein (CD47) (His tag)

Produktnummer ABIN7320544

Recombinant CD47-Protein exprimiert in Human Cells.

Kurzübersicht für CD47 Protein (CD47) (His tag) (ABIN7320544)

Target: CD47

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Human Cells

Reinheit: > 95 % as determined by SDS-PAGE

Produktdetails

Aufreinigungstag / Konjugat: Dieses CD47 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse CD47 Protein (His Tag) (Active)

Sequenz: Gln19-Pro158

Produktmerkmale: Recombinant Mouse Leukocyte surface antigen CD47 is produced by our Mammalian expression system and the target gene encoding Gln19-Pro158 is expressed with a 6His tag at the C-terminus.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Mouse SIRPA -Fc(Cat: PKSM041145) at 2μg/ml(100 μl/well) can bind Mouse CD47-His. The ED50 of Mouse CD47-His is 5.736 ug/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: CD47

Andere Bezeichnung: CD47

Hintergrund:

Background: CD47, also known as Integrin-Associated Protein (IAP) and OA3, is a glycosylated atypical member of the immunoglobulin superfamily. Mouse CD47 is an integral membrane protein that consists of a extracellular domain (ECD) with a single Ig-like domain, five membrane-spanning regions with short intervening loops, and C-terminal cytoplasmic tail. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It plays an important role in memory formation and synaptic plasticity in the hippocampus. As a receptor for SIRPA, it binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cellcell adhesion, it enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. It may play a role in membrane transport and/or integrin dependent signal transduction. It also prevents premature elimination of red blood cells.

Synonym: LAP,OA3,Leukocyte Surface Antigen CD47, Antigenic Surface Determinant Protein OA3, Integrin-Associated Protein,Protein MER6, CD47, MER6

Molekulargewicht: 16.7 kDa