CD28 Protein (CD28) (His tag)

Produktnummer ABIN7319757

Dieses Recombinant CD28-Protein wird in Human Cells produziert.

Kurzübersicht für CD28 Protein (CD28) (His tag) (ABIN7319757)

Target: CD28

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human, Cynomolgus

Quelle: Human Cells

Reinheit: >95 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses CD28 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human/Cynomolgus CD28/TP44 (N-His)

Sequenz: Asn19-Pro152

Produktmerkmale: Recombinant Human T-cell-specific Surface Glycoprotein CD28 is produced by our Mammalian expression system and the target gene encoding Asn19-Pro152 is expressed with a 6His tag at the N-terminus.

Endotoxin-Niveau: < 1 IEU/μg as determined by LAL test.

Biological Activity Comment: Immobilized  Human/Cynomolgus CD28-His(Cat#C406) at 2μg/ml (100 μl/well) can bind Anti-CD28 mAb(Cat#A063). The ED50 of  Anti-CD28 mAb(Cat#A063) is 15.3 ng/ml.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: CD28

Andere Bezeichnung: / CD28/TP44

Hintergrund:

Background: T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Synonym: CD28, CD28 antigen, CD28 Molecule, T-cell-specific surface glycoprotein CD28, Tp44, TP44

Molekulargewicht: 16.7 kDa

UniProt: P10747

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response