CSNK2A2 Protein (His-GST)

Produktnummer ABIN7317080

Recombinant CSNK2A2-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für CSNK2A2 Protein (His-GST) (ABIN7317080)

Target: CSNK2A2 (Casein Kinase 2, alpha Prime Polypeptide (CSNK2A2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 92 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses CSNK2A2 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human CSNK2A2/CK2A2 Protein (His & GST Tag)

Sequenz: Met 1-Arg 350

Produktmerkmale: A DNA sequence encoding the human CSNK2A2 (NP_001887.1) (Met 1-Arg 350) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 30 nmol/min/mg using casein as substrate.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile solution of 50 mM Tris, 500 mM NaCl, 0.5 mM PMSF, 0.5 mM TCEP, 10 % glycerol, pH 8.5

Konservierungsmittel: PMSF

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: CSNK2A2 (Casein Kinase 2, alpha Prime Polypeptide (CSNK2A2))

Andere Bezeichnung: CSNK2A2/CK2A2

Hintergrund: CK2A2,CSNK2A1,Casein kinase II subunit alpha', also known as CSNK2A2 and CK2A2, is a member of the protein kinase superfamily, Ser/Thr protein kinase family and CK2 subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. CSNK2A2 is a tetramer composed of an alpha chain, an alpha' and two beta chains. It is also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Protein kinase casein kinase II (Ck2) is a cyclic-AMP and calcium-independent serine-threonine kinase that is composed of two catalytic subunits (alpha and alpha') and two regulatory beta-subunits. Ck2 is not a casein kinase in vivo, but over 100 substrates are known. The highly conserved amino acid sequences of its subunits and their broad expression suggest that Ck2 may have a fundamental role in cell function. Ck2 has been implicated in DNA replication, regulation of basal and inducible transcription, translation and control of metabolism. The Ck2alpha and Ck2alpha' isoforms (products of the genes Csnk2a1 and Csnk2a2, respectively) are highly homologous, the reason for their redundancy and evolutionary conservation is unknown. CSNK2A2 may be a candidate gene for these inherited syndromes.

Molekulargewicht:

Calculated MW: 69.0 kDa

Observed MW: 60 kDa

Gen-ID: 1459

NCBI Accession: NP_001887

UniProt: P19784

Pathways: SARS-CoV-2 Protein Interaktom