BTD Protein (His tag)

Produktnummer ABIN7194393

Dieses Recombinant BTD-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für BTD Protein (His tag) (ABIN7194393)

Target: BTD (Biotinidase (BTD))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses BTD Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Biotinidase/BTD Protein (His Tag)

Sequenz: Met 1-Asp543

Produktmerkmale: A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to hydrolyze biocytin to lysine and biotin. The specific activity is > 500pmol/min/μg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: BTD (Biotinidase (BTD))

Andere Bezeichnung: Biotinidase/BTD

Hintergrund: BTD,Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing offats,carbohydrates, andproteins. Biotin is attached to these carboxylase enzymes through anamino acid(the building material of proteins) calledlysine, forming a complex calledbiocytin.

Molekulargewicht:

Calculated MW: 58.2 kDa

Observed MW: 66-76 kDa

Gen-ID: 686

UniProt: P43251