BFAR Protein (AA 1-183)

Produktnummer ABIN7321012

Recombinant BFAR-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für BFAR Protein (AA 1-183) (ABIN7321012)

Target: BFAR (Bifunctional Apoptosis Regulator (BFAR))

Protein-Typ: Recombinant

Spezies: Streptomyces hygroscopicus

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 1-183

Verwendungszweck: Recombinant Streptomyces hygroscopicus Bar Protein

Sequenz: Met1-Ile183

Produktmerkmale: Recombinant Streptomyces hygroscopicus Bar is produced by our E.coli expression system and the target gene encoding Met1-Ile183 is expressed.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 12.5 mM Tris-HCl, 50 mM NaCl, 5 % Trehalose, 5 % Mannitol, 0.01 % Tween 80, 2 mM DTT, 1 mM EDTA, pH 8.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: BFAR (Bifunctional Apoptosis Regulator (BFAR))

Andere Bezeichnung: Bar

Hintergrund: PPT N-acetyltransferase,Phosphinothricin N-acetyltransferase,Phosphinothricin-resistance protein,bar,Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.

Molekulargewicht:

Calculated MW: 20.6 kDa

Observed MW: 18-20 kDa

UniProt: P16426