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BACE1 Protein

Recombinant BACE1-Protein exprimiert in HEK-293 Cells.
Produktnummer ABIN7318053

Kurzübersicht für BACE1 Protein (ABIN7318053)

Target

Alle BACE1 Proteine anzeigen
BACE1 (Beta-secretase 1 (BACE1))

Protein-Typ

Recombinant

Biologische Aktivität

Active

Spezies

  • 13
  • 4
  • 3
  • 1
  • 1
Human

Quelle

  • 8
  • 8
  • 3
  • 1
  • 1
  • 1
HEK-293 Cells

Reinheit

> 95 % as determined by reducing SDS-PAGE.
  • Verwendungszweck

    Recombinant Human BACE1/ASP2 Protein (Active)

    Sequenz

    Met 1-Thr 457

    Produktmerkmale

    The mature form of human BACE1 (NP_036236.1) extracellular domain (Met1-Thr 457) with a quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.

    Endotoxin-Niveau

    < 1.0 EU per μg as determined by the LAL method.

    Biological Activity Comment

    Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2 (Catalog# ES004, R&D Systems). Cleavage of ES004 can be measured using excitation and emission wavelength of 320 and 405 nm, respectively. The specific activity is >3.5 pmoles/min/μg.
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  • Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Please refer to the printed manual for detailed information.

    Buffer

    Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0

    Lagerung

    4 °C,-20 °C,-80 °C

    Informationen zur Lagerung

    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Target

    BACE1 (Beta-secretase 1 (BACE1))

    Andere Bezeichnung

    BACE1

    Hintergrund

    Background: Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.

    Synonym: ASP2,BACE,HSPC104

    Molekulargewicht

    49 kDa

    NCBI Accession

    NP_036236
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