ENPP7 Protein (His tag)

Produktnummer ABIN7194214

Dieses Recombinant ENPP7-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für ENPP7 Protein (His tag) (ABIN7194214)

Target: ENPP7 (Alkaline Sphingomyelinase (Alk-Smase))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses ENPP7 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human ENPP7/NPP-7 Protein (His Tag)

Sequenz: Met 1-Ser 439

Produktmerkmale: A DNA sequence encoding the human ENPP7 (NP_848638.2) (Met 1-Ser 439) was fused with a C-terminal polyhistidine tag.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: ENPP7 (Alkaline Sphingomyelinase (Alk-Smase))

Andere Bezeichnung: ENPP7/NPP-7

Hintergrund:

Background: Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.

Synonym: ALK-SMase;E-NPP7;NPP-7;NPP7

Molekulargewicht: 49 kDa

NCBI Accession: NP_848638