Aconitase 1 Protein (ACO1) (His tag)

Produktnummer ABIN7194099

Dieses Recombinant Aconitase 1-Protein wird in Baculovirus infected Insect Cells produziert.

Kurzübersicht für Aconitase 1 Protein (ACO1) (His tag) (ABIN7194099)

Target: Aconitase 1 (ACO1)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses Aconitase 1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human ACO1/irp1 Protein (His Tag)

Sequenz: Met 1-Lys 889

Produktmerkmale: A DNA sequence encoding the human ACO1 (P21399) (Met 1-Lys 889) was expressed, with a polyhistidine tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10 % glycerol, 2 mM DTT
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: Aconitase 1 (ACO1)

Andere Bezeichnung: ACO1/irp1

Hintergrund: ACONS,HEL60,IREB1,IREBP,IREBP1,IRP1,Aconitase 1(ACO1) or IRP1 is one member of the aconitase family that contains a diverse group of iron-sulphur(Fe-S) isomerases and two types of iron regulatory protein. Aconitase exits in two forms: one is soluble and the other is mitochondrial. ACO1 is the soluble existing form, and the mitochondrial form is ACO2. Residues from all three N-terminal domains and the larger C-terminal domain contribute to the active site region. When the enzyme is activated, it gains an additional iron atom. ACO1 can assume two different functions in cells, depending on different conditions. During iron scarcity or oxidative stress, ACO1 binds to mRNA stem-loop structures called iron responsive elements to modulate the translation of iron metabolism genes. In iron-rich conditions, ACO1 binds an iron-sulfur cluster to function as a cytosolic aconitase.

Molekulargewicht:

Calculated MW: 101 kDa

Observed MW: 90 kDa

Gen-ID: 48

UniProt: P21399

Pathways: Transition Metal Ion Homeostasis