PHAP1 Protein (His-GST)

Produktnummer ABIN7317458

Recombinant PHAP1-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für PHAP1 Protein (His-GST) (ABIN7317458)

Target: PHAP1 (ANP32A) (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 85 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses PHAP1 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human ANP32A/PHAP1 Protein (His & GST Tag)

Sequenz: Glu 2-Lys 238

Produktmerkmale: A DNA sequence encoding the of human ANP32A (NP_006296.1) (Glu 2-Lys 238) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 10 % glycerol, 1 mM DTT, 0.5 mM GSH
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: PHAP1 (ANP32A) (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A))

Andere Bezeichnung: ANP32A/PHAP1

Hintergrund: C15orf1,HPPCn,I1PP2A,LANP,MAPM,PHAP1,PHAPI,PP32,acidic leucine-rich nuclear phosphoprotein 32 family member A, also known as acidic nuclear phosphoprotein pp32, Leucine-rich acidic nuclear protein, Mapmodulin, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, Putative HLA-DR-associated protein I, PHAPI and ANP32A, is a nucleus, cytoplasm and endoplasmic reticulum. ANP32A / LANP is expressed in all tissues tested. It is highly expressed in kidney and skeletal muscle, moderate levels of expression is in brain, placenta and pancreas. ANP32A / LANP is weakly expressed in lung. It is found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala. ANP32A / LANP is a component of the SET complex, which also contains SET, APEX1, HMGB2 and NME1. It directly interacts with SET. ANP32A / LANP also interacts with ATXN1/SCA1. ANP32A / LANP is implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. ANP32A / LANP plays a role in E4F1-mediated transcriptional repression.

Molekulargewicht:

Calculated MW: 55.4 kDa

Observed MW: 50 kDa

Gen-ID: 8125

NCBI Accession: NP_006296

UniProt: P39687