AACS Protein (His tag)

Produktnummer ABIN7317651

Dieses Recombinant AACS-Protein wird in Baculovirus infected Insect Cells exprimiert.

Kurzübersicht für AACS Protein (His tag) (ABIN7317651)

Target: AACS (Acetoacetyl-CoA Synthetase (AACS))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 96 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses AACS Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human AACS/Acetoacetyl-CoA Synthetase Protein (His Tag)

Sequenz: Met 1-Phe 672

Produktmerkmale: A DNA sequence encoding the full length of human AACS isoform a (NP_076417.2) (Met 1-Phe 672) was expressed, with a polyhistidine tag at the N-terminus.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: AACS (Acetoacetyl-CoA Synthetase (AACS))

Andere Bezeichnung: AACS/Acetoacetyl-CoA Synthetase

Hintergrund:

Background: Acetoacetyl-CoA Synthetase (AACS) is a novel cytosolic ketone body (acetoacetate)-specific ligase. The AACS in adipose tissue plays an important role in utilizing ketone body for the fatty acid-synthesis during adipose tissue development. It had been improved that Acetoacetyl-CoA Synthetase is an essential enzyme for the synthesis of fatty acid and cholesterol from ketone bodies, was found to be highly expressed in mouse adipose tissue, and GC box and C/EBPs motif were crucial for AACS promoter activity in 3T3-L1 adipocytes. Moreover, AACS promoter activity was controlled mainly by C/EBPalpha during adipogenesis. AACS gene expression is particularly abundant in white adipose tissue, as it is induced during adipocyte differentiation. The human AACS promoter is a PPARgamma target gene and that this nuclear receptor is recruited to the AACS promoter by direct interaction with Sp1 (stimulating protein-1). The Acetoacetyl-CoA Synthetase has important roles in the regulation of ketone body utilization in rat liver and that these hypocholesterolemic agents have the ability to remedy the impaired utilization of ketone bodies under the diabetic condition.

Synonym: ACSF1,SUR-5

Molekulargewicht: 77 kDa

NCBI Accession: NP_076417

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis