PFKFB3 Protein (His-GST)

Produktnummer ABIN7194084

Dieses Recombinant PFKFB3-Protein wird in Baculovirus infected Insect Cells produziert.

Kurzübersicht für PFKFB3 Protein (His-GST) (ABIN7194084)

Target: PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 85 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses PFKFB3 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human PFK2/PFKFB3 Protein (His & GST Tag)

Sequenz: Met 1-His 520

Produktmerkmale: A DNA sequence encoding the human PFKFB3 isoform 1 (Q16875-1) (Met 1-His 520) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 7.0, 10 % glycerol, 0.3 mM DTT

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))

Andere Bezeichnung: PFK2/PFKFB3

Hintergrund: IPFK2,PFK2,Fructose-2,6-biphosphatase 3, also known as 6-phosphofructo-2-kinase or PFK2 or PFKFB3, is a potent activator of phosphofructokinase, which is a rate-limiting enzyme of glycolysis. Highly phosphorylated PFKFB3 protein was found in human tumor cells, vascular endothelial cells, and smooth muscle cells. Fructose 2,6-bisphosphate (Fru-2,6-BP) is an allosteric activator of 6-phosphofructo-1-kinase (PFK-1), a rate-limiting enzyme and essential control point in glycolysis. The concentration of PFK2 depends on the activity of the bifunctional enzyme, 6-phosphofructo-2-kinase / fructose-2,6-bisphosphatase (PFK-2 / FBPase). PFK2 controls the glycolytic flux via the allosteric activator fructose 2,6-bisphosphate. Because of its proto-oncogenic character, the PFK-2/FBPase-2 of the PFKFB3 gene is assumed to play a critical role in tumorigenesis. The hypoxia-inducible form of 6-phosphofructo-2-kinase / fructose-2,6-bisphosphatase (PFKFB3) plays a crucial role in the progression of cancerous cells by enabling their glycolytic pathways even under severe hypoxic conditions.

Molekulargewicht:

Calculated MW: 87.4 kDa

Observed MW: 75 kDa

Gen-ID: 5209

UniProt: Q16875

Pathways: AMPK Signaling, Regulation of Carbohydrate Metabolic Process