LRRC32&TGF-beta protein (His tag)

Produktnummer ABIN7125972

Dieses Recombinant LRRC32&TGF-beta-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für LRRC32&TGF-beta protein (His tag) (ABIN7125972)

Target: LRRC32&TGF-beta

Protein-Typ: Recombinant

Spezies: Cynomolgus

Quelle: HEK-293 Cells

Reinheit: >90 % as determined by SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: His tag

Verwendungszweck: Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free

Sequenz: Ala 18 -Asn 627 (LRRC32) & Leu 30 - Ser 390 (TGF-beta 1)

Produktmerkmale: Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free is expressed from human 293 cells (HEK293). It contains AA Ala 18 -Asn 627 (LRRC32) & Leu 30 - Ser 390 (TGF-beta 1) (Accession # A0A2K5X2X9-1 (LRRC32) & A0A2K5TJB2-1 (TGF-beta 1).

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: Cynomolgus LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free (the molar ratio of LRRC32 & 1 equals 1:2) is produced by co-expression of LRRC32 and 1, which has a calculated MW of 68.1 kDa (LRRC32), 28.5 (LAP) and 12.8 kDa (mature 1) respectively. LRRC32 is fused with a polyhistidine tag at the C-terminus and 1 contains no tag. The reducing (R) Heterotrimer protein migrates as 70 kDa (LRRC32), 37-43 kDa (LAP) and 13 kDa (mature 1) due to glycosylation respectively.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Antigendetails

Target: LRRC32&TGF-beta

Hintergrund: Synonyms: LRRC32 & TGF-beta 1,LRRC32&TGFB1,
GARP (LRRC32) is a transmembrane protein that binds latent-TGF-β1 and tethers it on the Treg surface. and has been proved to promote the activation and secretion of transforming growth factor β (TGF-β). The expression of GARP is highly on the surface activated Tregs and increases the suppressive function of Tregs. Additionally, GARP can bind to latent transforming growth factor β (TGF-β), thus promoting secretion and activation of TGF-β. TGF-β plays a critical rule for homeostasis and function of Tregs. Notably, it has been also observed that fibroblasts and endothelial cell lines that express GARP/latent TGF-β1 complexes do not activate TGF-β1. However, it cannot be excluded that specific stimuli are required to trigger TGF-β1 activation from complexes on the surface of these cell types.

Molekulargewicht: 68.1 kDa (LRRC32) & 41.2 kDa (TGF-beta 1)