SPAM1 Protein

Produktnummer ABIN7199566

Produktdetails

Target: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Verwendungszweck: Human PH20 / SPAM1 Protein, Tag Free (MALS verified)

Sequenz: Leu 36 - Ser 490

Produktmerkmale: Human PH20, Tag Free (PH0-H5219) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Ser 490 (Accession # P38567-1).

Reinheit: >95 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Güteklasse: MALS verified

Anwendungsinformationen

Applikationshinweise: This protein carries no "tag". The protein has a calculated MW of 52.6 kDa. The protein migrates as 65-70 kDa under reducing (R) condition due to glycosylation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM NaCl, pH 7.5

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Antigendetails

Target: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Andere Bezeichnung: / SPAM1 (SPAM1 Produkte)

Hintergrund: Synonyms: SPAM1,PH-20,HYAL3,HYA1,HYAL1,HYAL5,SPAG15,
Description: Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N - acetyl - beta - D - glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Molekulargewicht: 52.6 kDa

NCBI Accession: NP_694859