CD99 Protein (CD99) (His-Avi Tag,Biotin)

Produktnummer ABIN7199186

Dieses Recombinant CD99-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für CD99 Protein (CD99) (His-Avi Tag,Biotin) (ABIN7199186)

Target: CD99

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: >90 % as determined by SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses CD99 Protein ist gelabelt mit His-Avi Tag,Biotin.

Verwendungszweck: Biotinylated Human CD99 / MIC2 Protein, His,Avitag™

Sequenz: Asp 23 - Asp 122

Produktmerkmale: Biotinylated Human CD99, His,Avitag (CD9-H82E7) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 122 (Accession # P14209-1).

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 13.7 kDa. The protein migrates as 22 kDa and 27 kDa under reducing (R) condition due to glycosylation.

Kommentare:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Antigendetails

Target: CD99

Andere Bezeichnung: CD99 / MIC2

Substanzklasse: Viral Protein

Hintergrund: Synonyms: CD99,HBA71,MIC2,MIC2X,MIC2Y,MSK5X,12E7,
Description: CD99 antigen is also known as MIC2, single-chain type-1 glycoprotein, HBA71, MIC2X, MIC2Y, MSK5X, 12E7, which belongs to the CD99 family. CD99 / MIC2 is expressed on all leukocytes but highest on thymocytes. Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 / MIC2 plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. CD99 acts at the same site as, but independently of PECAM1. There is also experimental evidence that it binds to cyclophilin A.

Molekulargewicht: 13.7 kDa