CD38 Protein (Fc-Avi Tag,Biotin)

Produktnummer ABIN7199090

Dieses Recombinant CD38-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für CD38 Protein (Fc-Avi Tag,Biotin) (ABIN7199090)

Target: CD38

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: >95 % as determined by SDS-PAGE.

Güteklasse: MALS verified

Produktdetails

Aufreinigungstag / Konjugat: Dieses CD38 Protein ist gelabelt mit Fc-Avi Tag,Biotin.

Verwendungszweck: Biotinylated Human CD38 Protein, Fc,Avitag™ (MALS verified)

Sequenz: Val 43 - Ile 300

Produktmerkmale: Biotinylated Human CD38, Fc,Avitag (CD8-H82F5) is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300 (Accession # P28907-1).

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 58.0 kDa. The protein migrates as 65-75 kDa under reducing (R) condition due to glycosylation.

Kommentare:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Antigendetails

Target: CD38

Hintergrund: Synonyms: CD38,T10,cADPr hydrolase 1,
Description: CD antigen CD38 is also known as ADP-ribosyl cyclase 1, which belongs to the ADP-ribosyl cyclase family. CD38 is expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. These reaction products are essential for the regulation of intracellular Ca2+. The loss of CD38 function is associated with impaired immune responses, metabolic disturbances, and behavioral modifications. The CD38 protein is a marker of cell activation. It has been connected to HIV infection, leukemias, myelomas, solid tumors, type II diabetes mellitus and bone metabolism. CD38 has been used as a prognostic marker in leukemia.

Molekulargewicht: 58.0 kDa

NCBI Accession: NP_001766