MBP Protein (AA 99-330) (Fc Tag)

Produktnummer ABIN7092845

Dieses Recombinant MBP-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für MBP Protein (AA 99-330) (Fc Tag) (ABIN7092845)

Target: MBP

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Reinheit: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Produktdetails

Proteineigenschaft: AA 99-330

Aufreinigungstag / Konjugat: Dieses MBP Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Recombinant human MBP Protein with N-terminal Human Fc tag

Spezifität: HFc(Glu99-Ala330) MBP(Met1-Arg304)

Produktmerkmale: Extracellular Domain Protein

Aufreinigung: Purified from cell culture supernatant by affinity chromatography

Anwendungsinformationen

Applikationshinweise: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Kommentare:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: MBP

Hintergrund: Myelin A1 protein,Myelin membrane encephalitogenic protein
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]

Molekulargewicht: predicted molecular mass of 59.3 kDa after removal of the signal peptide. The apparent molecular mass of hFc-MBP is 70-100 kDa due to glycosylation.

UniProt: P02686