SARS-CoV-2 NSP1 Protein (His tag)

Produktnummer ABIN6952638

Produktdetails

Target: SARS-CoV-2 NSP1 (NSP1) (SARS-CoV-2 Host Translation Inhibitor Nsp1 (NSP1))

Protein-Typ: Recombinant

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 NSP1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Verwendungszweck: SARS-CoV-2 (COVID-19) NSP1 Protein, His Tag

Sequenz: AA 1-180

Produktmerkmale: SARS-CoV-2 NSP1, His Tag is expressed from E.coli cells. It contains AA Met 1 - Gly 180 (Accession # YP_009725297.1). Predicted N-terminus: Met
This protein carries a polyhistidine tag at the C-terminus.

Reinheit: >90 % as determined by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, Arginine, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.

Antigendetails

Target: SARS-CoV-2 NSP1 (NSP1) (SARS-CoV-2 Host Translation Inhibitor Nsp1 (NSP1))

Andere Bezeichnung: SARS-CoV-2 Host Translation Inhibitor Nsp1 (NSP1 Produkte)

Substanzklasse: Viral Protein

Hintergrund: The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.

Molekulargewicht: 21.7 kDa

NCBI Accession: YP_009725297