SNCA Protein (A53T)

Produktnummer ABIN6952303

Dieses Recombinant SNCA-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für SNCA Protein (A53T) (ABIN6952303)

Target: SNCA (Synuclein, alpha (SNCA))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Western Blotting (WB), In vitro Assay (in vitro), In vivo Studies (in vivo)

Produktdetails

Proteineigenschaft: A53T

Sequenz: MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Spezifität: ~14.46 kDa

Produktmerkmale: Active Human Recombinant A53T Mutant Alpha Synuclein Protein Preformed Fibrils (Type 1)

Aufreinigung: Ion-exchange Purified

Biological Activity Comment: 100 μM A53T alpha synuclein protein monomer (SPR-325) seeded with 10 nM A53T alpha synuclein protein PFF (SPR-326) in 25 μM Thioflavin T (PBS pH 7.4, 100 μl reaction volume) generated a fluorescence intensity of 28 000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 56 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.

Anwendungsinformationen

Kommentare:

Certified >95% pure using SDS-PAGE analysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: Lot specific

Buffer: PBS pH 7.4

Lagerung: -80 °C

Antigendetails

Target: SNCA (Synuclein, alpha (SNCA))

Andere Bezeichnung: Alpha Synuclein

Hintergrund: Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals. Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus. Functionally, it has been shown to significantly interact with tubulin, and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions, inactivation may lead to impaired spatial learning and working memory. SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin. The A53T mutation is a missense point mutation where alanine is replaced by threonine at the 53rd amino acid. This mutation has been linked to early-onset Parkinson's Disease and increased rates of alpha synuclein fibrillization.

Gen-ID: 6622

NCBI Accession: NP_000336

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity