CXCL12 Protein (AA 24-89) (Biotin)

Produktnummer ABIN6952225

Produktdetails

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 24-89

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses CXCL12 Protein ist gelabelt mit Biotin.

Applikation: Flow Cytometry (FACS), Cell Migration Assay (CMA)

Sequenz: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

Produktmerkmale: Biotininylated Recombinant Human CXCL12 (SDF-1alpha)

Reinheit: > 97 % by SDS PAGE

Endotoxin-Niveau: <0.01 EU per 1μg of the protein by the LAL method

Biological Activity Comment: EC50 = 0.5-0.9 nM determined by Calcium Flux with human CXCR4 in U937 cells
Migration confirmed with U937 cells expressing CXCR4

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Spin tube prior to resuspending. Recommended at 100 μg/mL in sterile water

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: 12 months from date of receipt, -20 to -70 °C as supplied.
Suggest to use immediately after reconstitution
1 month at -20 to -70 °C under sterile conditions after reconstitution.

Haltbarkeit: 12 months

Antigendetails

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Andere Bezeichnung: CXCL12/SDF-1alpha (CXCL12 Produkte)

Hintergrund: Stromal-Cell Derived Factor-1alpha (SDF-1alpha) (CXCL12) attracts lymphocytes and plays important roles in embryogenesis and angiogenesis with implications in tumor metastasis. By binding to CXCR4, one of the co-receptors for HIV viral entry, SDF-1alpha can suppress HIV. Besides CXCR4, SDF-1alpha also binds to the “decoy” receptor CXCR7, and activates the downstream beta-arrestin- rather than G protein-mediated signaling pathway.

Molekulargewicht: 10.381 kDa

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling