Human Adenovirus type 11 (HAdV-11) Protein

Produktnummer ABIN6941930

Dieses Recombinant Human Adenovirus type 11-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für Human Adenovirus type 11 (HAdV-11) Protein (ABIN6941930)

Target: Human Adenovirus type 11 (HAdV-11)

Protein-Typ: Recombinant

Spezies: Adenovirus

Quelle: HEK-293 Cells

Reinheit: 0.99%

Produktdetails

Verwendungszweck: Wild type Adenovirus type 11. Concentrated and purified virus particles from a double CsCl gradient purification with DNase treatment and dialysis. Safety category BSL2.

Produktmerkmale: Adenovirus Type 11 Particles, Wild-type

Aufreinigung: Human adenovirus type 11 particles (wild-type) produced in HEK293 cells and purified on CsCl gradient.

Anwendungsinformationen

Kommentare:

This product is a concentrated source of highly-purified human Adenovirus type 11 particles (wild-type) from a lysate of optimally-infected 293 cells. Following double CsCl gradient purification with DNase treatment and dialysis, our Ad11 preparation is of very high quality, with minimal lot-to-lot variation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, 50 mM Hepes pH 7.8, 7.5 % Sucrose

Lagerung: -80 °C

Informationen zur Lagerung: -80°C

Antigendetails

Target: Human Adenovirus type 11 (HAdV-11)

Andere Bezeichnung: Adenovirus Type 11

Substanzklasse: Virus

Hintergrund: Adenoviruses are medium-sized (80–100 nm), non-enveloped viruses. They have an icosahedral nucleocapsid containing a linear, double-stranded DNA genome of approximately 36 kb (Nermut, 1984). The viral genome is grouped into different transcriptional units, designated early (E1, E2, E3, E4), intermediate, and late. The E1 gene is essential for activation of other viral genes and for viral replication. Deletion of the E1 gene results in viruses that are replication incompetent in normal cells. However, replication-competent viral particles can be produced from E1-deleted viral vectors by providing the E1 gene in trans. The E3 gene is nonessential for either viral replication or infection (Flint, 1999).
Adenovirus type 11 (Ad11) is associated with respiratory tract infections and conjunctivitis. It belongs to the B group of adenoviruses (along with Ad3), which use a different host cell receptor to Ad5 (Ad5 belongs to group C). Ad11 uses CD46 (and other receptors), whereas Ad5 binds the Coxsackie and adenovirus receptor (CAR). CAR expression is often down-regulated in human tumors, making Ad5-mediated gene transfer problematic. This has resulted in the development of chimeric Ad5/Ad11 vectors for improved oncolytic gene therapy.