Human Adenovirus type 3 (HAdV-3) Protein

Produktnummer ABIN6941928

Recombinant Human Adenovirus type 3-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für Human Adenovirus type 3 (HAdV-3) Protein (ABIN6941928)

Target: Human Adenovirus type 3 (HAdV-3)

Protein-Typ: Recombinant

Spezies: Adenovirus

Quelle: HEK-293 Cells

Reinheit: Purified virus

Produktdetails

Verwendungszweck: Wild type Adenovirus type 3. Concentrated and purified virus particles from a double CsCl gradient purification with DNase treatment and dialysis. Safety category BSL2.

Produktmerkmale: Adenovirus Type 3 Particles, Wild-type
Human adenovirus type 3 particles (wild-type) produced in HEK293 cells and purified by CsCl gradient.

Aufreinigung: Human adenovirus type 3 particles (wild-type) produced in HEK293 cells and purified by CsCl gradient.

Anwendungsinformationen

Applikationshinweise: 2.53x10^12 particles per mL (BCA assay)

Kommentare:

This product is a concentrated source of highly-purified human Adenovirus type 3 particles (wild-type) from a lysate of optimally-infected 293 cells. Following double CsCl gradient purification with DNase treatment and dialysis, this Ad3 preparation is of very high quality and minimal lot-to-lot variation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, 50 mM Hepes pH 7.8, 10 % Glycerol

Lagerung: -80 °C

Informationen zur Lagerung: -80°C

Antigendetails

Target: Human Adenovirus type 3 (HAdV-3)

Andere Bezeichnung: Adenovirus Type 3

Substanzklasse: Virus

Hintergrund: Adenoviruses are medium-sized (80–100 nm), non-enveloped viruses. They have an icosahedral nucleocapsid containing a linear, double-stranded DNA genome of approximately 36 kb (Nermut, 1984). The viral genome is grouped into different transcriptional units, designated early (E1, E2, E3, E4), intermediate, and late. The E1 gene is essential for activation of other viral genes and for viral replication. Deletion of the E1 gene results in viruses that are replication incompetent in normal cells. However, replication-competent viral particles can be produced from E1-deleted viral vectors by providing the E1 gene in trans. The E3 gene is nonessential for either viral replication or infection (Flint, 1999).