EBI3 Protein

Produktnummer ABIN6700897

Hersteller Produkt- Nr.: 010-001-b66

Dieses Recombinant EBI3-Protein wird in Escherichia coli (E. coli) produziert. Es ist 1 Publikation verfügbar.

Kurzübersicht für EBI3 Protein (ABIN6700897)

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 90,00%

Produktdetails

Hersteller Produkt- Nr.: 010-001-b66

Hersteller: Rockland

Verwendungszweck: EBI-3 Mouse Recombinant Protein

Aufreinigung: Recombinant protein corresponding to amino acids 2 to 207 of mature mouse EBI-3. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC, each against a known standard.

Endotoxin-Niveau: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: Assay data for mouse recombinant EBI-3 is based upon qualitative binding to anti-EBI-3 antibody.

Anwendungsinformationen

Applikationshinweise:

Application Note: EBI-3 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-EBI-3 in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: 0.02M HCl

Reconstitution_Volume: 100 μL

Konzentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Referenzen

Nikolic, Ruiz-Garrido, Crespo, Romero-Becerra, Leiva-Vega, Mora, León, Rodríguez, Leiva, Plata-Gómez, Alvarez Flores, Torres, Hernández-Cosido, López, Vázquez, Efeyan, Martin, Marcos, Sabio: "Lack of p38 activation in T cells increases IL-35 and protects against obesity by promoting thermogenesis." in: EMBO reports, Vol. 25, Issue 6, pp. 2635-2661, (2024) (PubMed).

Antigendetails

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Andere Bezeichnung: Ebi3

Hintergrund:

Synonyms: Interleukin-27 subunit beta precursor [Mus musculus], mouse EBI-3 cytokine, EBI3, EBI-3, IL35, IL-35

Background: Epstein-Barr Virus Induced Gene-3 (EBI-3), is a secreted glycoprotein belonging to the hematopoietin receptor family and related to the p40 subunit of IL-12. It was identified by its induced expression in B-lymphocytes in response to Epstein-Barr virus infection. EBI-3 forms heterodimers with p28 to form IL-27 and with p35 to form IL-35. Both IL-27 and IL-35 have anti-inflammatory and regulatory activity. Recombinant Mouse EBI is a non-glycosylated polypeptide chain consisting of 207 amino acids with a molecular weight of 22,900 Da.

Gen-ID: 50498

UniProt: O35228