Vimentin Protein (VIM) (His tag)

Produktnummer ABIN6700879

Hersteller Produkt- Nr.: 009-001-e54s

Dieses Recombinant Vimentin-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für Vimentin Protein (VIM) (His tag) (ABIN6700879)

Target: Vimentin (VIM)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB)

Reinheit: >95%

Produktdetails

Aufreinigungstag / Konjugat: Dieses Vimentin Protein ist gelabelt mit His tag.

Hersteller Produkt- Nr.: 009-001-e54s

Hersteller: Rockland

Verwendungszweck: Vimentin recombinant protein-HIS Epitope

Aufreinigung: Recombinant full-length human Vimentin was expressed by E. coli cells using an N-terminal his epitope. The purity was determined to be >95% by densitometry.

Anwendungsinformationen

Applikationshinweise:

Application Note: Vimentin Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. Vimentin Protein is suitable for use in Western Blot. Expect a band approximately ~60 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 μg/μL

Buffer: Vimentin Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Vimentin (VIM)

Andere Bezeichnung: VIM

Hintergrund:

Synonyms: VIM, FLJ36605, Vimentin

Background: Vimentin is a member of intermediate filament family of proteins and is an important structural feature of eukaryotic cells (1). Along with microtubules and actin microfilaments, Vimentin make up the cytoskeleton component of cells. Studies have shown that Vimentin is attached to the nucleus, endoplasmic reticulum and mitochondria, either laterally or terminally. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol (2). Thus, Vimentin plays a key role in maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vimentin Protein is ideal for investigators involved in Signaling Proteins, Microtubule/Actin Associated Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Inflammation, Invasion/Metastasis, and Neurobiology research.

NCBI Accession: NM_003380

Pathways: Caspase Kaskade in der Apoptose