SIRT5 Protein (GST tag)

Produktnummer ABIN6700535

Hersteller Produkt- Nr.: 009-001-t35s

Dieses Recombinant SIRT5-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für SIRT5 Protein (GST tag) (ABIN6700535)

Target: SIRT5 (Sirtuin 5 (SIRT5))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB)

Reinheit: >95%

Produktdetails

Aufreinigungstag / Konjugat: Dieses SIRT5 Protein ist gelabelt mit GST tag.

Hersteller Produkt- Nr.: 009-001-t35s

Hersteller: Rockland

Verwendungszweck: SIRT5 recombinant protein-GST fusion protein

Aufreinigung: Full-length recombinant human SIRT5 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Anwendungsinformationen

Applikationshinweise:

Application Note: SIRT5 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. SIRT5 Protein is suitable for use in Western Blot. Expect a band approximately ~60 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 μg/μL

Buffer: SIRT5 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: SIRT5 (Sirtuin 5 (SIRT5))

Andere Bezeichnung: SIRT5

Hintergrund:

Synonyms: SIR2L5, Sirtuin 5, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2-like protein 5, SIRT5, SIR2L5

Background: SIRT5 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT5 being a member of class III. SIRT5 consists of eight exons and is found in two isoforms which encode a 310 aa and a 299 aa protein, respectively. Human SIRT5 is most predominantly expressed in heart muscle cells and in lymphoblasts. Fluorescence in situ hybridization analysis localized the human SIRT5 gene to chromosome 6p23. SIRT5 can deacetylate cytochrome c, a protein of the mitochondrial intermembrane space with a central function in oxidative metabolism as well as apoptosis initiation (1). SIRT5 Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.

NCBI Accession: NM_012241

Pathways: SARS-CoV-2 Protein Interaktom