SIRT4 Protein (GST tag)

Produktnummer ABIN6700533

Hersteller Produkt- Nr.: 009-001-t34s

Produktdetails

Target: SIRT4 (Sirtuin 4 (SIRT4))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses SIRT4 Protein ist gelabelt mit GST tag.

Applikation: Western Blotting (WB)

Hersteller Produkt- Nr.: 009-001-t34s

Hersteller: Rockland

Verwendungszweck: SIRT4 recombinant protein-GST fusion protein

Aufreinigung: Full-length recombinant human SIRT4 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

Reinheit: >70%

Anwendungsinformationen

Applikationshinweise:

Western_Blot_Dilution: User Optimized

Application_Note: SIRT4 Protein is suitable for use in Western Blot. Expect a band approximately ~62 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 μg/μL

Buffer: SIRT4 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: SIRT4 (Sirtuin 4 (SIRT4))

Andere Bezeichnung: SIRT4 (SIRT4 Produkte)

Hintergrund:

Synonyms: IR2L4, MGC57437, MGC130046, MGC130047, sirtuin 4, NAD-dependent protein deacetylase sirtuin-4, NAD-dependent ADP-ribosyltransferase sirtuin-4, Regulatory protein SIR2 homolog 4, SIR2-like protein 4, SIRT4, SIR2L4

Background: SIRT4 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT4 being a member of class IV. SIRT4 lacks deacetylase activity but has ADP-ribosyltransferase activity (1). Immunoprecipitation analysis shows that SIRT4 interacts with the mitochondrial enzyme glutamate dehydrogenase (GDH), and functional analysis show that SIRT4 ADP-ribosylates and inhibits GDH (2). Downregulation of SIRT4 by RNA interference activates GDH, thereby upregulating insulin secretion in response to glucose and amino acids. SIRT4 Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.

NCBI Accession: NM_012240

Pathways: Negative Regulation of Hormone Secretion