PAKRK2 protein (GST tag)

Produktnummer ABIN6700344

Hersteller Produkt- Nr.: 009-001-s80s

Recombinant PAKRK2-Protein exprimiert in Insect cells (Sf9).

Kurzübersicht für PAKRK2 protein (GST tag) (ABIN6700344)

Target: PAKRK2

Protein-Typ: Recombinant

Spezies: Human

Quelle: Insect cells (Sf9)

Applikation: Western Blotting (WB)

Reinheit: >75%

Produktdetails

Aufreinigungstag / Konjugat: GST tag

Hersteller Produkt- Nr.: 009-001-s80s

Hersteller: Rockland

Verwendungszweck: Parkin recombinant protein-GST fusion protein

Aufreinigung: Recombinant full-length human Parkin was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.

Anwendungsinformationen

Applikationshinweise:

Application Note: Parkin Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. Parkin Protein is suitable for use in Western Blot. Expect a band approximately ~68 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 μg/μL

Buffer: Parkin Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: PAKRK2

Hintergrund:

Synonyms: PARK2, AR-JP, PDJ, PRKN, LPRS2,E3 ubiquitin-protein ligase parkin

Background: Parkin or parkinson protein 2 is a RING domain-containing E3 ubiquitin protein ligase involved in proteasome-dependent degradation of proteins. Mutations in Parkin can lead to Parkinson's disease. Parkin is important for mitochondrial quality control by lysosome-dependent degradation of damaged mitochondria through autophagy, or mitophagy (1). Parkin plays an important role in directly supporting mitochondrial function and protecting mitochondrial genomic integrity from oxidative stress (2). Parkin also has essential role in regulating normal respiratory function in mitochondria and in the protection of cells from oxidative stress. PARK7 Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Apoptosis/Autophagy, Cancer, Cell Cycle, and Neurobiology research.