NAMPT Protein

Produktnummer ABIN6700313

Hersteller Produkt- Nr.: 009-001-w40-0005

Dieses Recombinant NAMPT-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für NAMPT Protein (ABIN6700313)

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 90,00%

Produktdetails

Hersteller Produkt- Nr.: 009-001-w40-0005

Hersteller: Rockland

Verwendungszweck: Human Visfatin Recombinant Protein

Aufreinigung: Visfatin purity was determined to be greater than 90% as determined by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by its ability to induce IL-6, IL-1 beta and TNF alpha production from human PBMCs at 100 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Visfatin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Visfatin in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: 0.02M HCl

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Andere Bezeichnung: NAMPT

Hintergrund:

Synonyms: Pre-B-cell colony-enhancing factor 1, PBEF

Background: Visfatin is an adipokine produced by visceral adipose tissue. Other adipokines include leptin, adiponectin, resistin, chemerin, omentin and vaspin. Visfatin has been shown to act as a pro-inflammatory factor for PBMCs and may mimic the effects of insulin on adipocytes, monocytes and hepatocytes. Recombinant human Visfatin is a non-glycosylated protein, containing 466 amino acids, with a molecular weight of 52.6 kDa.

UniProt: P43490