IL17F Protein

Produktnummer ABIN6700054

Hersteller Produkt- Nr.: 010-001-b31

Dieses Recombinant IL17F-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für IL17F Protein (ABIN6700054)

Target: IL17F (Interleukin 17F (IL17F))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 97,00%

Produktdetails

Hersteller Produkt- Nr.: 010-001-b31

Hersteller: Rockland

Verwendungszweck: IL-17F Mouse Recombinant Protein

Aufreinigung: Purity was determined to be greater than 97% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.

Endotoxin-Niveau: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: IL-17F is fully bioactive when compared to standard. The ED50 as determined by the dose-dependant induction of IL-6 in NHDF is 1.94 ng/ml.

Anwendungsinformationen

Applikationshinweise:

Application Note: IL-17F protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-17F in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 25 μL (25-250 μL)

Konzentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: IL17F (Interleukin 17F (IL17F))

Andere Bezeichnung: Il17f

Hintergrund:

Synonyms: Interleukin-17F cytokine, IL-17F, Interleukin-24, IL-24, Cytokine ML-1

Background: Recombinant Mouse IL-17F produced in E.coli is a homodimeric, non-glycosylated polypeptide chain, the monomer containing a total of 133 amino acids and having a molecular mass of 14.9 kDa. Homodimeric interleukin-17F is a member of the IL-17 family of six homodimeric proteins (IL-17A-17F). Activated CD4+ T cells have also been shown to produce Mouse IL-17F. Activity studies reveal IL-17A to be the most potent, followed by IL-17A/F and IL-17F 100 fold lower than IL-17A. Recombinant Mouse IL-17F produced in E.Coli is a non-glycosylated polypeptide comprised of two monomeric subunit each of mIL-17F. The dimer consists of 266 amino acids, with an approximate molecular weight of 29.8 kDa.

UniProt: Q7TNI7

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity