IFNG1-2 Protein

Produktnummer ABIN6699978

Hersteller Produkt- Nr.: 010-001-v20-0020

Recombinant IFNG1-2-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für IFNG1-2 Protein (ABIN6699978)

Target: IFNG1-2 (Interferon, gamma 1-2 (IFNG1-2))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 95,00%

Produktdetails

Hersteller Produkt- Nr.: 010-001-v20-0020

Hersteller: Rockland

Verwendungszweck: Mouse Interferon gamma Recombinant Protein

Aufreinigung: Interferon gamma purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by Viral CPE assay using EMC virus on L929 cells and is typically greater than 1.0 x 10^7units/mg.

Anwendungsinformationen

Applikationshinweise:

Application Note: Interferon-gamma Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Interferon-gamma in immunological assays. Product is provided as a dried powder, lyophilized from 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer: Product is provided as a dried powder, lyophilized from 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: IFNG1-2 (Interferon, gamma 1-2 (IFNG1-2))

Andere Bezeichnung: INF gamma

Hintergrund:

Synonyms: Type II interferon, T cell interferon, Immune Interferon, MAF

Background: Interferon gamma, IFNγ, is a major immune-modulating molecule produced mainly by T cells and natural killer cells activated by antigens, mitogens or alloantigens. Most immune cells express IFNγ receptors and respond to IFNγ-induced signaling by up-regulating MHC class I expression. Human IFNγ does not show cross-reactivity with mouse. Recombinant mouse IFNγ is a non-glycosylated protein, containing 134 amino acids, with a molecular weight of 15.6 kDa.

UniProt: P01580