HSPA1AB protein (GST tag)

Produktnummer ABIN6699933

Hersteller Produkt- Nr.: 009-001-f63s

Recombinant HSPA1AB-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für HSPA1AB protein (GST tag) (ABIN6699933)

Target: HSPA1AB

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB)

Reinheit: >90%

Produktdetails

Aufreinigungstag / Konjugat: GST tag

Hersteller Produkt- Nr.: 009-001-f63s

Hersteller: Rockland

Verwendungszweck: HSP70 recombinant protein-GST fusion protein

Aufreinigung: Recombinant full-length human HSP70 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Anwendungsinformationen

Applikationshinweise:

Application Note: HSP70 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. HSP70 Protein is suitable for use in Western Blot and ATPase Assay. Expect a band approximately ~96 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Other: ATPase Assay-User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 μg/μL

Buffer: HSP70 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: HSPA1AB

Hintergrund:

Synonyms: HSPA1A, HSP72, HSPA1, HSPA1B, HSP70-1, Heat shock 70 kDa protein 1A/1B , Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP70.1/HSP70.2

Background: HSP70 is a member of the heat shock protein family and is synthesized by cells of many organisms in response to stress (1). HSP70 is found mostly but not exclusively in the nucleus of unstressed cells. For several hours after a short heat shock, however, it is strongly concentrated in nucleoli (2). Nucleoli are transiently damaged by such a heat shock: their morphology changes and assembly and export of ribosomes is blocked for several hours. HSP70 helps to stabilize the morphological changes of the nucleoli. HSP70 Protein is ideal for investigators involved in Signaling Proteins, Cell Stress & Chaperone Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cellular Stress, and Inflammation research.

NCBI Accession: NM_005345