GBR2 protein (GST tag)

Produktnummer ABIN6699916

Hersteller Produkt- Nr.: 009-001-r99s

Dieses Recombinant GBR2-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für GBR2 protein (GST tag) (ABIN6699916)

Target: GBR2

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB)

Reinheit: >95%

Produktdetails

Aufreinigungstag / Konjugat: GST tag

Hersteller Produkt- Nr.: 009-001-r99s

Hersteller: Rockland

Verwendungszweck: GRB2 recombinant protein-GST fusion protein

Aufreinigung: Recombinant full-length human GRB2 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Anwendungsinformationen

Applikationshinweise:

Application Note: GRB2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. GRB2 Protein is suitable for use in Western Blot. Expect a band approximately ~49 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 μg/μL

Buffer: GRB2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: GBR2

Hintergrund:

Synonyms: ASH, Grb3-3, MST084, MSTP084, EGFRBP-GRB2, Growth factor receptor-bound protein 2, Adapter protein GRB2, Protein Ash, SH2/SH3 adapter GRB2

Background: GRB2 is an adaptor protein that is the homolog of the Sem5 gene of C. elegans and Drk gene of Drosophila. GRB2 protein contains one SH2 domain and two SH3 domains and plays a role in growth factor-mediated signal transduction. GRB2 forms a complex with activated EGFR and the RAS-specific guanine nucleotide exchange factor SOS1 thereby mediating the growth factor-induced activation of RAS (1). GRB2 has been implicated in growth factor regulation of the cytoskeleton and DNA synthesis. DAB2 also binds to GRB2 and competes with SOS for GRB2 binding. GRB2 interacts with the hepatitis C virus nonstructural 5A (NS5A) protein and inhibits the activation of ERK1 and ERK2 by EGF (2). GRB2 Protein is ideal for investigators involved in Signaling Proteins, Adaptor Proteins, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, JAK/STAT Pathway, and Neurobiology research.

NCBI Accession: NM_002086