Cyclin O Protein (CCNO) (GST tag)

Produktnummer ABIN6699749

Hersteller Produkt- Nr.: 009-001-r82s

Dieses Recombinant Cyclin O-Protein wird in Insect cells (Sf9) exprimiert.

Kurzübersicht für Cyclin O Protein (CCNO) (GST tag) (ABIN6699749)

Target: Cyclin O (CCNO)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Insect cells (Sf9)

Applikation: Western Blotting (WB)

Reinheit: >95%

Produktdetails

Aufreinigungstag / Konjugat: Dieses Cyclin O Protein ist gelabelt mit GST tag.

Hersteller Produkt- Nr.: 009-001-r82s

Hersteller: Rockland

Verwendungszweck: CyclinO recombinant protein-GST fusion protein

Aufreinigung: Recombinant full-length human CyclinO was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Anwendungsinformationen

Applikationshinweise:

Application Note: CyclinO Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. CyclinO Protein is suitable for use in Western Blot. Expect a band approximately ~ 69 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 μg/μL

Buffer: CyclinO Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Cyclin O (CCNO)

Andere Bezeichnung: CCNO

Hintergrund:

Synonyms: CCNO, CCNU, UDG2, Cyclin-O

Background: Cyclin O belongs to the highly conserved cyclin family. These family members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclin O plays an important role in the processes of transcription. Cyclin O also plays an important role in DNA repair and cell cycle progression. Cyclin O localizes exclusively in the nucleus (1). Cyclin O shows significant uracil-DNA glycosylase activity in which the activity of in vitro translated UDG2 was not as robust as that of the mitochondrial isoform of UNG (2). Cyclin O Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.

NCBI Accession: NM_021147