Cyclin K Protein (CCNK) (GST tag)

Produktnummer ABIN6699747

Hersteller Produkt- Nr.: 009-001-r81s

Produktdetails

Target: Cyclin K (CCNK)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Insect cells (Sf9)

Aufreinigungstag / Konjugat: Dieses Cyclin K Protein ist gelabelt mit GST tag.

Applikation: Western Blotting (WB)

Hersteller Produkt- Nr.: 009-001-r81s

Hersteller: Rockland

Verwendungszweck: CyclinK recombinant protein-GST fusion protein

Aufreinigung: Recombinant full-length human CyclinK was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

Reinheit: >85%

Anwendungsinformationen

Applikationshinweise:

Western_Blot_Dilution: User Optimized

Application_Note: CyclinK Protein is suitable for use in Western Blot. Expect a band approximately ~ 67 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 μg/μL

Buffer: CyclinK Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Cyclin K (CCNK)

Andere Bezeichnung: CCNK (CCNK Produkte)

Hintergrund:

Synonyms: CCNK, CPR4, MGC9113, Cyclin-K

Background: CyclinK is a member of the cyclin family which can regulate transcription through their association with and activation of cyclin-dependent kinases (CDK). The CDK/CyclinK complex can phosphorylate the C-terminal domain (CTD) of the large subunit of RNA polymerase II (1). CyclinK is associated with potent CTD kinase and CDK kinase activities in vitro and coimmunoprecipitates with POLR2A. CyclinK is a new member of the transcription cyclin family which may play a dual role in regulating CDK and RNA polymerase II activities (2). Cyclin K Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.