CX3CL1 Protein

Produktnummer ABIN6699702

Hersteller Produkt- Nr.: 009-001-h49-0005

Recombinant CX3CL1-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für CX3CL1 Protein (ABIN6699702)

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: 98,00%

Produktdetails

Hersteller Produkt- Nr.: 009-001-h49-0005

Hersteller: Rockland

Verwendungszweck: Human Fractalkine (CX3CL1) Recombinant Protein

Aufreinigung: Fractalkine (CX3CL1) purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose dependent chemotaxis of human PBMCs and is typically starting between 10-100 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Other: User Optimized

Application_Note: Fractalkine Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Fractalkine in immunological assays.

Kommentare:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Andere Bezeichnung: CX3CL1

Hintergrund:

Synonyms: C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1

Background: Fractalkine, also known as CX3CL1, is an atypical chemokine that was the first of a fourth chemokine motif (CX3C). It is thought to function as a T cell and monocyte chemotractant and is produced by non-haemopoietic cells. Fractalkine is made in a soluble and membrane bound form in activated endothelial cells which is thought to promote adhesion of leukocytes. Recombinant human Fractalkine is a non-glycosylated protein, containing 76 amino acids, with a molecular weight of 8.6 kDa.

UniProt: P78423

Pathways: Synaptic Membrane