ARTN Protein

Produktnummer ABIN6699532

Hersteller Produkt- Nr.: 009-001-u64-0100

Dieses Recombinant ARTN-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für ARTN Protein (ABIN6699532)

Target: ARTN (Artemin (ARTN))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 98,00%

Produktdetails

Hersteller Produkt- Nr.: 009-001-u64-0100

Hersteller: Rockland

Verwendungszweck: Human Artemin Recombinant Protein

Aufreinigung: Artemin purity was determined to be greater than 98% as determined by analysis by HpLC and by reducing and non-reducing SDS-pAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependant proliferation of the SH-SY5Y cell line and is typically 4-8 ng/mL. The activity can also be determined by its ability to promote survival and neurite outgrowth.

Anwendungsinformationen

Applikationshinweise:

Application Note: Recombinant human Artemin has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Artemin in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: ARTN (Artemin (ARTN))

Andere Bezeichnung: ARTN

Hintergrund:

Synonyms: ARTN, enovin, neublastin

Background: Artemin is a novel member of the glial cell line-derived neurotrophic factor (GNDF) ligand family. Current evidence suggests that Artemin signals through the receptor complex, GFRα3-RET, to influence neuron survival in vitro and in vivo. Recombinant human Artemin is a non-glycosylated, disulfide-linked homodimer, containing two 113 amino acid chains, with a total molecular weight of 24.2 kDa.

UniProt: Q5T4W7