Ppa (AA 1-176) protein (His tag)

Produktnummer ABIN667500

Produktdetails

Target: Ppa

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-176

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: His tag

Applikation: SDS-PAGE (SDS)

Produktmerkmale: ppa, 1-176aa, E.coli, His tag, E.coli

Reinheit: > 95 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl

Lagerung: 4 °C

Antigendetails

Target: Ppa

Hintergrund: Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations. Recombinant E.coli ppa protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: inorganic pyrophosphatase, ECK4222, JW4185. NCBI no.: NP_418647

Molekulargewicht: 21.9kDa (196aa), confirmed by MALDI-TOF