AnsA (AA 1-338) protein (His tag)

Produktnummer ABIN667462

Produktdetails

Target: AnsA

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-338

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: His tag

Applikation: SDS-PAGE (SDS)

Produktmerkmale: ansA, 1-338aa, E.coli, His tag, E.coli

Reinheit: > 95 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT.

Lagerung: 4 °C

Antigendetails

Target: AnsA

Hintergrund: AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Escherichia coli possesse two l-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB). AnsA (Type I asparaginase) is constitutively expressed and required for the growth of the bacteria on asparagine as the sole nitrogen source. Recombinant E.coli ansA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Cytoplasmic L-asparaginase I, ECK1765, JW1756. NCBI no.: NP_416281

Molekulargewicht: 39.3 kDa (358aa), confirmed by MALDI-TOF