NTH (AA 1-211) protein (His tag)

Produktnummer ABIN667447

Produktdetails

Target: NTH

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-211

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: His tag

Applikation: SDS-PAGE (SDS)

Produktmerkmale: nth, 1-211aa, E.coli, His tag, E.coli

Reinheit: > 85 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF

Lagerung: 4 °C

Antigendetails

Target: NTH

Hintergrund: Nth, also known as Endonuclease III, has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme incises damaged DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5' from the damaged site. This enzyme is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. Recombinant E.coli nth protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: Endonuclease III, b1633, JW1625, DNA-(apurinic or apyrimidinic site) lyase. NCBI no.: NP_416150

Molekulargewicht: 25.7 kDa (231aa) confirmed by MALDI-TOF