GrxB (AA 1-215) protein (His tag)

Produktnummer ABIN667442

Produktdetails

Target: GrxB

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-215

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: His tag

Applikation: SDS-PAGE (SDS)

Produktmerkmale: grxB, 1-215aa, E.coli, His tag, E.coli

Reinheit: > 95 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl

Lagerung: 4 °C

Antigendetails

Target: GrxB

Hintergrund: GrxB (Glutaredoxin-2) belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes of approximately one hundred amino-acid residues that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. This protein involved in reducing some disulfides in a coupled system with glutathione reductase. It does not act as hydrogen donor for ribonucleotide reductase. Recombinant E.coli grxB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: glutaredoxin 2, Grx2, bA101E13.1, CGI-133, GRLX2. NCBI no.: NP_415582

Molekulargewicht: 26.5kDa (235aa) confirmed by MALDI-TOF