NanA (AA 1-297) protein (His tag)

Produktnummer ABIN667434

Produktdetails

Target: NanA

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-297

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: His tag

Applikation: SDS-PAGE (SDS)

Produktmerkmale: nanA, 1-297aa, E.coli, His tag, E.coli

Reinheit: > 95 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol

Lagerung: 4 °C

Antigendetails

Target: NanA

Hintergrund: NanA, also known as N-acetylneuraminate lyase, belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. NanA catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetyl-D-mannosamine. This protein was inhibited by reduction with NaBH4 in the presence of the substrate, indicating that it belongs to the Schiff-base-forming Class I aldolases. NanA was strongly inhibited by Cu2+ ions, p-chloromercuribenzoate and N-bromosuccinimide, and also inhibited competitively by the reaction product, pyruvate, and its structurally related compounds, dihydroxyacetone and DL-glyceraldehyde. Recombinant E.coli nanA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: N-acetylneuraminate lyase, npl. NCBI no.: NP_417692

Molekulargewicht: 34.7 kDa (317aa) confirmed by MALDI-TOF