HchA (AA 1-283) protein (His tag)

Produktnummer ABIN667426

Produktdetails

Target: HchA

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-283

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: His tag

Applikation: SDS-PAGE (SDS)

Produktmerkmale: hchA, 1-283aa, E.coli, His tag, E.coli

Reinheit: > 95 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/ml (determined by Bradford assay)

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Lagerung: 4 °C

Antigendetails

Target: HchA

Hintergrund: HchA, also known as, Escherichia coli Hsp31, is a homodimeric member of the ThiI/DJ-1/PfpI superfamily that combines molecular chaperone and aminopeptidase activities. It uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress. Recombinant E. coli hchA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Hsp31 molecular chaperone, ECK1963, JW1950, yedU, yzzC. NCBI no.: NP_416476

Molekulargewicht: 33.3 kDa(303aa) confirmed by MALDI-TOF